Trolleybus lines 25 and 26 will operate only in the section Osová / Kamenný vrch - Provazníkova and will then divert to the stop Štefánikova čtvrť.

Trolleybus line 27 will be introduced to serve Vinohrady and Líšeň on the route Stará osada - Pálavské náměstí - Novolíšeňská - Jírova.

Bus line 53 will be limited in intervals during peak hours on weekdays and connections to the Štefánikova čtvrť will only go to the Halasovo náměstí stop. The operation of line 53 will be supplemented by the newly introduced bus line 72.

Bus line 57 will be extended from Merhautova Street along the route of lines 25 and 26 via Tomkovo Square to Pálavské Square. Selected connections to Obřany will be cancelled (replaced by the newly introduced bus line 72). Buses of line 57 will not go to Stará Osada. Bus lines 44 and 84 can be used to travel between Tomek Square and Stará Osada.

On peak weekdays bus line 72 will be in operation on the route Technologický park - Záhřebská - Královo Pole, žel.st. - Halasovo náměstí - Štefánikova čtvrt' - Tomkovo náměstí - Obřany, sídliště. In the section Technologický park - Štefánikova čtvrt' (Štefánikova čtvrť), line 72 will replace part of line 53. In the section Tomkovo náměstí - Obřany, sídliště, line 72 will replace the diverted connections of line 57.

Transfer between lines 25, 26, 44, 57, 72 and 84 will be possible at the Štefánikova čtvrt' stop.