Due to the construction works, tram traffic in the part of Benešova Street between the Křenová viaduct and the Grandhotel Brno will be excluded from the morning departures on Monday 30 June 2025 until the completion of the works (presumably by the end of August 2025).

Due to construction works, from Monday 30 June 2025 until the completion of the works (assumed 13 December 2025), traffic will be restricted in Jihlavská Street between the junctions with Netroufalky and Dlouha Streets.

Trolleybus line 25 will operate only in the section Nemocnice Bohunice - Líšeň, Jírova.

- For the section Osová - Nemocnice Bohunice it will be possible to use regular bus and tram lines.

The Stará nemocnice stop of lines 40 and N90 in the direction from the centre will be relocated to a temporary platform in the central dividing strip at the level of the regular stop.